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使用光子芯片的高通量总内反射荧光和直接随机光学重建显微镜
JoVE Journal
生化学
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JoVE Journal 生化学
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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使用光子芯片的高通量总内反射荧光和直接随机光学重建显微镜

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14:09 min

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November 16, 2019

DOI:

10.3791/60378-v

DOI:
10.3791/60378-v

•

14:09 min
November 16, 2019

•

9 Views
, , , ,
1Department of Physics and Technology,UiT The Arctic University of Norway, 2Vascular Biology Research Group, Department of Medical Biology,UiT The Arctic University of Norway

概要

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基于芯片的超分辨率光学显微镜是荧光显微镜的一种新方法，具有成本效益和吞吐量方面的优势。此处显示了用于 TIRF 显微镜和基于定位的超分辨率显微镜的芯片制备和成像协议。

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Cite this Article

Coucheron, D. A., Helle, Ø. I., Øie, C. I., Tinguely, J., Ahluwalia, B. S. High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip. J. Vis. Exp. (153), e60378, doi:10.3791/60378 (2019).

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