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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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Biochemie
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JoVE Journal Biochemie
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

6,577 Views

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14:09 min

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November 16, 2019

DOI:

10.3791/60378-v

DOI:
10.3791/60378-v

•

14:09 min
November 16, 2019

•

9 Views
, , , ,
1Department of Physics and Technology,UiT The Arctic University of Norway, 2Vascular Biology Research Group, Department of Medical Biology,UiT The Arctic University of Norway

Summary

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Chip-based super-resolution optical microscopy is a novel approach to fluorescence microscopy and offers advantages in cost effectiveness and throughput. Here, the protocols for chip preparation and imaging are shown for TIRF microscopy and localization-based super-resolution microscopy.

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Coucheron, D. A., Helle, Ø. I., Øie, C. I., Tinguely, J., Ahluwalia, B. S. High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip. J. Vis. Exp. (153), e60378, doi:10.3791/60378 (2019).

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