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In Vivo Optical Calcium Imaging of Learning-Induced Synaptic Plasticity in Drosophila melanogaster
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JoVE Journal 神経科学
In Vivo Optical Calcium Imaging of Learning-Induced Synaptic Plasticity in Drosophila melanogaster

In Vivo Optical Calcium Imaging of Learning-Induced Synaptic Plasticity in Drosophila melanogaster

DOI:
10.3791/60288-v

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06:35 min

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October 08, 2019

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, ,
1Department of Molecular Neurobiology of Behavior,University of Göttingen

章

  • 00:04標題
  • 00:26In Vivo Calcium Imaging Preparation
  • 02:44In Vivo Calcium Imaging
  • 03:39Olfactory Conditioning and Odor-Evoked Calcium Transients Visualization
  • 05:07Results: Representative Postsynaptically-Localized Calcium and Learning-Induced Postsynaptic Plasticity Visualization
  • 05:56Conclusion

概要

自動翻訳

自動翻訳

Here we present a protocol with which pre- and/or postsynaptic calcium can be visualized in the context of Drosophila learning and memory. In vivo calcium imaging using synaptically localized calcium sensors is combined with a classical olfactory conditioning paradigm such that the synaptic plasticity underlying this type of associative learning may be determined.

タグ

In Vivo Optical Calcium ImagingSynaptic PlasticityDrosophila MelanogasterAssociative TrainingGenetically Encoded Calcium IndicatorImaging ChamberOdor DeliveryRinger’s SolutionDissectionCuticle RemovalNeuronal Responses

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