This protocol outlines the establishment of a mouse model for studying Malassezia-host interactions in the skin. It describes the cultivation of Malassezia in vitro, the infection of the murine skin with Malassezia, and the subsequent analysis of the inflammation and the fungal burden in the skin tissue.
Sparber, F., LeibundGut-Landmann, S. Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction. J. Vis. Exp. (153), e60175, doi:10.3791/60175 (2019).