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SUMO 结合实体 （SUBEs） 作为肝癌中 SUMO 蛋白酶的浓缩、分离、识别和表征的工具
JoVE Journal
生化学
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JoVE Journal 生化学
SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
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SUMO 结合实体 （SUBEs） 作为肝癌中 SUMO 蛋白酶的浓缩、分离、识别和表征的工具

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08:29 min

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November 01, 2019

DOI:

10.3791/60098-v

DOI:
10.3791/60098-v

•

08:29 min
November 01, 2019

•

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1Liver Disease and Liver Metabolism Lab,CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 2Proteomics Platforms,CIC bioGUNE, ProteoRED-ISCIII, CIBERehd, 3Advanced Technology Institute in Life Sciences (ITAV)-CNRS-IPBS,UbiCARE

概要

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在这里，我们提出了一个协议，以丰富、分离、识别和描述通过SUMO结合实体（SUBEs）从小鼠模型获得的从人肝细胞和肝肿瘤中获得的体内由SUMO修饰的蛋白质。

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Lopitz-Otsoa, F., Delgado, T. C., Lachiondo-Ortega, S., Azkargorta, M., Elortza, F., Rodríguez, M. S., Martínez-Chantar, M. L. SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer. J. Vis. Exp. (153), e60098, doi:10.3791/60098 (2019).

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