This content is Free Access.
Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR
8,248 Views
•
10:54 min
•
July 27, 2019
DOI:
概要
Automatically generated
This article outlines a simple PCR-based assay to monitor the activity of an active LINE-1 retrotransposon and to map de novo retrotranspositions in a given genome. Using the MCF7 cell line, we demonstrate herein how this method can be applied to detect activity of a LINE-1 located at 22q12.1.
Read Article
Cite this Article
Pradhan, B., Kauppi, L. Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR. J. Vis. Exp. (149), e59880, doi:10.3791/59880 (2019).
Copy