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Purification of a High Molecular Mass Protein in Streptococcus mutans
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JoVE Journal 免疫学と感染
Purification of a High Molecular Mass Protein in Streptococcus mutans

Purification of a High Molecular Mass Protein in Streptococcus mutans

DOI:
10.3791/59804-v

•

09:51 min

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September 14, 2019

•

, , , ,
1Department of Translational Research,Tsurumi University School of Dental Medicine, 2Laboratory for Oral Health Science

章

  • 00:06標題
  • 00:51PCR Amplification
  • 02:38Cell Transformation
  • 03:35Purification of Polyhistidine-tagged GTF-SI
  • 07:29Results: Generation of S. mutans His-gtfC and Functional Restoration
  • 08:59Conclusion

概要

自動翻訳

自動翻訳

Described here is a simple method for the purification of a gene product in Streptococcus mutans. This technique may be advantageous in the purification of proteins, especially membrane proteins and high molecular mass proteins, and can be used with various other bacterial species.

タグ

Protein PurificationStreptococcus MutansHigh Molecular Mass ProteinPCRGenomic DNA ExtractionGel ElectrophoresisGel ExtractionNested PCRElectroporationCompetent CellsBrain-heart Infusion Broth

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