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Pan-Lyssavirus real time RT-PCR voor rabiës diagnose
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
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Pan-Lyssavirus real time RT-PCR voor rabiës diagnose

20,701 Views

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06:25 min

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July 10, 2019

DOI:

10.3791/59709-v

DOI:
10.3791/59709-v

•

06:25 min
July 10, 2019

•

18 Views
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1Wildlife Zoonoses & Vector-Borne Diseases Research Group,Animal and Plant Health Agency, 2Institute of Infection and Global Health,University of Liverpool

概要

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Deze real-time RT-PCR met behulp van dsDNA intercalating Dye is geschikt om Lyssavirus infecties te diagnosticeren. De methode begint met RNA geëxtraheerd uit rabiës verdachte ante-mortem-of postmortemmonsters, detaillering Master Mix preparaat, RNA toevoeging, installatie van de real-time machine en juiste interpretatie van de resultaten.

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Cite this Article

Marston, D. A., Jennings, D. L., MacLaren, N. C., Dorey-Robinson, D., Fooks, A. R., Banyard, A. C., McElhinney, L. M. Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis. J. Vis. Exp. (149), e59709, doi:10.3791/59709 (2019).

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