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Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

20,720 Views

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06:25 min

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July 10, 2019

DOI:

10.3791/59709-v

DOI:
10.3791/59709-v

•

06:25 min
July 10, 2019

•

18 Views
, , , , , ,
1Wildlife Zoonoses & Vector-Borne Diseases Research Group,Animal and Plant Health Agency, 2Institute of Infection and Global Health,University of Liverpool

Summary

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This real-time RT-PCR using dsDNA intercalating dye is suitable to diagnose lyssavirus infections. The method begins with RNA extracted from rabies suspected ante-mortem or post-mortem samples, detailing master mix preparation, RNA addition, setup of the real-time machine and correct interpretation of results.

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Cite this Article

Marston, D. A., Jennings, D. L., MacLaren, N. C., Dorey-Robinson, D., Fooks, A. R., Banyard, A. C., McElhinney, L. M. Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis. J. Vis. Exp. (149), e59709, doi:10.3791/59709 (2019).

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