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Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
JoVE Journal
遺伝学
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JoVE Journal 遺伝学
Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

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07:11 min

May 13, 2019

DOI:

07:11 min
May 13, 2019

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概要

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This molecular-based approach for determining bacterial fitness facilitates precise and accurate detection of microorganisms using unique genomic DNA barcodes that are quantified via digital PCR. The protocol describes calculating the competitive index for Salmonella strains; however, the technology is readily adaptable to protocols requiring absolute quantification of any genetically-malleable organism.

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