このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
9,433 Views
•
07:11 min
•
May 13, 2019
DOI:
概要
Automatically generated
This molecular-based approach for determining bacterial fitness facilitates precise and accurate detection of microorganisms using unique genomic DNA barcodes that are quantified via digital PCR. The protocol describes calculating the competitive index for Salmonella strains; however, the technology is readily adaptable to protocols requiring absolute quantification of any genetically-malleable organism.
Explore More Videos
Digital PCRCompetitive IndexHigh-throughput AnalysisFitnessSalmonellaQuantificationMixed PopulationMolecular-based TechniquesGenetic MarkersBacterial ChromosomesGenomic DNADilution SeriesDigital PCR SupermixProbe-based ChemistryNo Template ControlsNegative ControlsPositive ControlsDroplet GeneratorThermal CyclerData Analysis Software
Read Article
Cite this Article
Shaw, J. A., Bourret, T. J. Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella. J. Vis. Exp. (147), e59630, doi:10.3791/59630 (2019).
Copy