このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
8,016 Views
•
09:45 min
•
May 21, 2019
DOI:
概要
Automatically generated
This protocol uses a probe-based real-time polymerase chain reaction (PCR), a sulforhodamine B (SRB) assay, 3’ untranslated regions (3’ UTR) cloning, and a luciferase assay to verify the target genes of a miRNA of interest and to understand the functions of miRNAs.
Read Article
Cite this Article
Seo, H. A., Hwang, C. Y., Moeng, S., Park, J. K. An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells. J. Vis. Exp. (147), e59628, doi:10.3791/59628 (2019).
Copy