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Extracción de metabolitos acuosos de células adherentes cultivadas para análisis metabolómicos por electroforesis capilar-espectrometría de masas
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がん研究
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Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
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Extracción de metabolitos acuosos de células adherentes cultivadas para análisis metabolómicos por electroforesis capilar-espectrometría de masas

7,868 Views

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11:39 min

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June 09, 2019

DOI:

10.3791/59551-v

DOI:
10.3791/59551-v

•

11:39 min
June 09, 2019

•

2 Views
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1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory,National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center,National Cancer Center

概要

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El propósito de este artículo es describir un protocolo para la extracción de metabolitos acuosos de células adherentes cultivadas para el análisis metabolómico, particularmente, electroforesis capilar-espectrometría de masas.

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Keyword Extraction:MetabolomicsCapillary Electrophoresis-mass SpectrometryCEMSAqueous MetabolitesCultured Adherent CellsCancer CellsMetabolite ExtractionBiomarkersChemotherapy ResponseCell CultureTrypsin-EDTACell SuspensionCentrifugation

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Cite this Article

Maruyama, A., Kami, K., Sasaki, K., Sato, H., Sato, Y., Tsuchihara, K., Makinoshima, H. Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry. J. Vis. Exp. (148), e59551, doi:10.3791/59551 (2019).

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