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Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

7,769 Views

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11:39 min

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June 09, 2019

DOI:

10.3791/59551-v

DOI:
10.3791/59551-v

•

11:39 min
June 09, 2019

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7 Views
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1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory,National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center,National Cancer Center

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Keyword Extraction:MetabolomicsCapillary Electrophoresis-mass SpectrometryCEMSAqueous MetabolitesCultured Adherent CellsCancer CellsMetabolite ExtractionBiomarkersChemotherapy ResponseCell CultureTrypsin-EDTACell SuspensionCentrifugation

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Maruyama, A., Kami, K., Sasaki, K., Sato, H., Sato, Y., Tsuchihara, K., Makinoshima, H. Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry. J. Vis. Exp. (148), e59551, doi:10.3791/59551 (2019).

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