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毛细管电泳质谱法提取培养细胞中的水代谢物进行代谢组学分析
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Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
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毛细管电泳质谱法提取培养细胞中的水代谢物进行代谢组学分析

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11:39 min

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June 09, 2019

DOI:

10.3791/59551-v

DOI:
10.3791/59551-v

•

11:39 min
June 09, 2019

•

2 Views
, , , , , ,
1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory,National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center,National Cancer Center

概要

Automatically generated

本文的目的是描述从培养的粘附细胞中提取水代谢物的方案, 用于代谢组学分析, 特别是毛细管电泳质谱法。

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Keyword Extraction:MetabolomicsCapillary Electrophoresis-mass SpectrometryCEMSAqueous MetabolitesCultured Adherent CellsCancer CellsMetabolite ExtractionBiomarkersChemotherapy ResponseCell CultureTrypsin-EDTACell SuspensionCentrifugation

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Maruyama, A., Kami, K., Sasaki, K., Sato, H., Sato, Y., Tsuchihara, K., Makinoshima, H. Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry. J. Vis. Exp. (148), e59551, doi:10.3791/59551 (2019).

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