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In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices
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In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices

In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices

DOI:
10.3791/59534-v

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09:07 min

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September 20, 2019

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, , ,
1CNRS (Integrative Neuroscience and Cognition Center, UMR 8002), 2Université Paris Descartes, Sorbonne Paris Cité

章

  • 00:04標題
  • 00:42Stereotaxic Surgery
  • 04:01Preparation of Brain Slices
  • 05:28Whole-Cell Patch-Clamp Recording
  • 07:21Results: Presubicular Layer III Neuron: Intrinsic Properties, Responses to Light Stimulation, and Post-hoc Revelation of Cell Morphology
  • 08:19Conclusion

概要

自動翻訳

自動翻訳

This protocol describes a set of methods to identify the cell-type specific functional connectivity of long-range inputs from distant brain regions using optogenetic stimulations in ex vivo brain slices.

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In VivoIntracerebralStereotaxicInjectionsOptogeneticStimulationLong-range InputsMouse Brain SlicesFunctional ConnectivityPhotostimulationAxonsStereotaxic TargetingLidocaine HydrochlorideCraniotomyViral SolutionHamilton Syringe

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