A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

Nasir Malik; Rohini Manickam; Muznabanu Bachani; Joseph P. Steiner

doi:10.3791/59333

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A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

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生化学

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JoVE Journal 生化学

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

8,372 Views

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00:12 min

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September 22, 2019

DOI:

10.3791/59333-v

DOI:

10.3791/59333-v

•

00:12 min

September 22, 2019

•

2 Views

Nasir Malik¹, Rohini Manickam¹, Muznabanu Bachani¹, Joseph P. Steiner¹

¹National Institute of Neurological Disorders and Stroke (NINDS), Neurotherapeutic Development Unit (NTDU),National Institutes of Health (NIH)

概要

It is often necessary to assess the potential cytotoxicity of a set of compounds on cultured cells. Here, we describe a strategy to reliably screen for toxic compounds in a 96-well format.

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Cell Growth Cell Survival Cell Culture Compound Screening Cytotoxicity Cell Proliferation Cell Viability High-throughput Low-to-moderate Throughput Multi-assay Strategy Cell Dissociation Cell Counting 96-well Plate Extracellular Matrix Coating Cell Culture Incubation

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A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

概要

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