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Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
JoVE Journal
生物学
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JoVE Journal 生物学
Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

14,262 Views

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12:58 min

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March 26, 2019

DOI:

10.3791/59288-v

DOI:
10.3791/59288-v

•

12:58 min
March 26, 2019

•

13 Views
, ,
1Core Imaging Facility and Department of Microbiology and Immunology,Loyola University Chicago, 2Department of Molecular Pharmacology and Therapeutics,Loyola University Chicago

概要

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We describe a comprehensive and practical protocol for fluorescence recovery after photobleaching experiments with live cells. Although the protocol was used to measure the mobility of yellow fluorescent protein-tagged p62 in aggresome-like induced structures, it can be applied to a variety of microscopy systems and fluorescent proteins.

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Cite this Article

Rademacher, D. J., Cabe, M., Bakowska, J. C. Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures. J. Vis. Exp. (145), e59288, doi:10.3791/59288 (2019).

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