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Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting
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生物学
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JoVE Journal 生物学
Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting

Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting

DOI:
10.3791/59269-v

•

07:55 min

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February 12, 2019

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1Research Programs Unit, Molecular Neurology,University of Helsinki

章

  • 00:04標題
  • 00:25Preparation of Mitochondrial Lysates
  • 02:40Preparation of Gradient Gel for BN-PAGE
  • 05:32Blue Native Gel Electrophoresis
  • 06:40Results: Investigating the Assembly of Mitochondrial OXPHOS Complexes Using BN-PAGE
  • 07:16Conclusion

概要

自動翻訳

自動翻訳

This protocol demonstrates the analysis of mitochondrial respiratory chain complexes by blue native polyacrylamide gel electrophoresis. Here the method applied to cultured human cells is described.

タグ

MitochondrialRespiratory ChainComplexesCulturedHumanCellsBlue NativePolyacrylamideGelElectrophoresisImmunoblottingOxidativePhosphorylationSystemIn-tactComplexesAssemblyPBSDigitoninProteaseInhibitorMitochondrialBufferLorealMountGradientGelPAGE

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