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Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR

Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR

DOI:

06:06 min

May 23, 2019

,

  • 00:04標題
  • 00:40Non-Obese Diabetic (NOD) Scid Gamma (NSG) Mouse Irradiation and Peripheral Blood Mononuclear Cell (PBMC) Injection
  • 02:22Retro-Orbital Injection
  • 03:24Clinical Graft Versus Host Disease (GVHD) Scoring
  • 03:58Genomic DNA Isolation and Human T Cell Quantification
  • 04:59Results: Representative GVHD Disease Progression and Digital PCR Human T Cell Detection
  • 05:37Conclusion

概要

自動翻訳

Here, we present a protocol to induce and score disease in a xenogeneic graft-versus-host disease (xenoGVHD) model. xenoGVHD provides an in vivo model to study immunosuppression of human T cells. Additionally, we describe how to detect human T cells in tissues with digital PCR as a tool to quantify immunosuppression.

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