This manuscript describes a simple method to measure stress behaviorally in adult zebrafish. The approach takes advantage of the innate tendency that zebrafish prefer the bottom half of a tank when in a stressful state. We also describe methods for coupling the assay with pharmacology.
Chin, J. S., Albert, L. T., Loomis, C. L., Keene, A. C., Duboué, E. R. Behavioral Approaches to Studying Innate Stress in Zebrafish. J. Vis. Exp. (147), e59092, doi:10.3791/59092 (2019).