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使用自动微生物反应器系统从中国仓鼠卵巢细胞中纯化和分析单克隆抗体

JoVE Journal
生物工学

This content is Free Access.

JoVE Journal 生物工学

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

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使用自动微生物反应器系统从中国仓鼠卵巢细胞中纯化和分析单克隆抗体

13,982 Views

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10:50 min

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May 01, 2019

DOI:

10.3791/58947-v

DOI:

10.3791/58947-v

•

10:50 min

May 01, 2019

•

16 Views

Sai Rashmika Velugula-Yellela¹, David N. Powers¹, Phillip Angart¹, Anneliese Faustino¹, Talia Faison¹, Casey Kohnhorst¹, Erica J. Fratz-Berilla¹, Cyrus D. Agarabi¹

¹Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II,U.S. Food and Drug Administration

概要

介绍了从自动微生物反应器的收获细胞培养液(HCCF)中分离和后续分析的单克隆抗体的详细方案。还介绍了使用分析来确定关键质量属性 (CQA) 和最大化有限样本量以提取重要信息。

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