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Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

13,967 Views

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10:50 min

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May 01, 2019

DOI:

10.3791/58947-v

DOI:
10.3791/58947-v

•

10:50 min
May 01, 2019

•

16 Views
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1Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II,U.S. Food and Drug Administration

Summary

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A detailed protocol for the purification and subsequent analysis of a monoclonal antibody from harvested cell culture fluid (HCCF) of automated microbioreactors has been described. Use of analytics to determine critical quality attributes (CQAs) and maximizing limited sample volume to extract vital information is also presented.

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Monoclonal AntibodyChinese Hamster Ovary CellsAutomated Microbioreactor SystemPurificationAnalyticsCritical Quality AttributesN-glycan LabelingLiquid Chromatography Mass Spectrometry (LC-MS)

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Cite this Article

Velugula-Yellela, S. R., Powers, D. N., Angart, P., Faustino, A., Faison, T., Kohnhorst, C., Fratz-Berilla, E. J., Agarabi, C. D. Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System. J. Vis. Exp. (147), e58947, doi:10.3791/58947 (2019).

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