Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Graeden Winkelaar; Catharine Trieber; Jitendra Kumar; Michael Overduin

doi:10.3791/58537

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Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

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Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

11,807 Views

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10:28 min

•

November 02, 2018

DOI:

10.3791/58537-v

DOI:

10.3791/58537-v

•

10:28 min

November 02, 2018

•

11 Views

Graeden Winkelaar¹, Catharine Trieber¹, Jitendra Kumar¹, Michael Overduin¹

¹Department of Biochemistry,University of Alberta

概要

Here, we present a protocol for the production and purification of proteins that are labeled with stable isotopes, and subsequent characterization of protein-protein interactions using Nuclear Magnetic Resonance (NMR) spectroscopy and MicroScale Thermophoresis (MST) experiments.

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Protein Interaction NMR Spectroscopy Microscale Thermophoresis MST Atomic-level Interaction Protein Binding Small Molecule Large Molecule Proton NMR SOFAST HMBC Magnetic Field Shimming Receiver Gain Adjustment Automatic Tuning Phase Correction Baseline Correction

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Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

概要

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