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Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo
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JoVE Journal 神経科学
Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo

Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo

6,915 Views

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11:01 min

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June 28, 2019

DOI:

10.3791/58503-v

DOI:
10.3791/58503-v

•

11:01 min
June 28, 2019

•

3 Views
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1Section on Neuroadaptation and Protein Metabolism, National Institute of Mental Health,National Institutes of Health (NIH)

概要

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Protein synthesis is a critical biological process for cells. In brain, it is required for adaptive changes. Measurement of rates of protein synthesis in the intact brain requires careful methodological considerations. Here we present the L-[1-14C]-leucine quantitative autoradiographic method for determination of regional rates of cerebral protein synthesis in vivo.

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Cite this Article

Saré, R. M., Torossian, A., Rosenheck, M., Huang, T., Beebe Smith, C. Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo. J. Vis. Exp. (148), e58503, doi:10.3791/58503 (2019).

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