このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities
12,282 Views
•
11:44 min
•
October 02, 2018
DOI:
概要
Automatically generated
Cysteine-rich peptides fold into distinct three-dimensional structures depending on their disulfide connectivity. Targeted synthesis of individual disulfide isomers is required when buffer oxidation does not lead to the desired disulfide connectivity. The protocol deals with the selective synthesis of 3-disulfide-bonded peptides and their structural analysis using NMR and MS/MS studies.
Read Article
Cite this Article
Heimer, P., Schmitz, T., Bäuml, C. A., Imhof, D. Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities. J. Vis. Exp. (140), e58368, doi:10.3791/58368 (2018).
Copy