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A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9
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生化学
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JoVE Journal 生化学
A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9

A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9

7,984 Views

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08:14 min

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August 28, 2018

DOI:

10.3791/58265-v

DOI:
10.3791/58265-v

•

08:14 min
August 28, 2018

•

3 Views
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1Division of Cardiology, Department of Medicine,Zuckerberg San Francisco General and University of California San Francisco, 2Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute,University of California San Francisco

概要

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This protocol presents a method to evaluate the proteolytic activity of an intrinsically low-activity, single turnover protease in a cellular context. Specifically, this method is applied to evaluate the proteolytic activity of PCSK9, a key driver of lipid metabolism whose proteolytic activity is required for its ultimate hypercholesterolemic function.

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Cite this Article

Chorba, J. S., Galvan, A. M., Shokat, K. M. A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9. J. Vis. Exp. (138), e58265, doi:10.3791/58265 (2018).

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