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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
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August 16, 2018
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Transient heterologous expression of biosynthetic enzymes in Nicotiana benthamiana leaves can divert endogenous supplies of 2,3-oxidosqualene towards the production of new high-value triterpene products. Herein is described a detailed protocol for rapid (5 days) preparative-scale production of triterpenes and analogs utilizing this powerful plant-based platform.
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Transient ExpressionNicotiana BenthamianaTriterpene ProductionPreparative ScaleAgrobacterium TumefaciensCo-infiltrationVacuum InfiltrationMMA BufferOD 600Preparative-scale AccessStructural CharacterizationMedicinally Relevant Biological ActivitySelective LB AgarSelective LB MediaCentrifugationResuspensionInfiltration Suspension
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Stephenson, M. J., Reed, J., Brouwer, B., Osbourn, A. Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale. J. Vis. Exp. (138), e58169, doi:10.3791/58169 (2018).
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