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Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
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生化学
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JoVE Journal 生化学
Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

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09:11 min

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October 02, 2018

DOI:

10.3791/58166-v

DOI:
10.3791/58166-v

•

09:11 min
October 02, 2018

•

17 Views
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1Unidad de Bioquímica y Biología Molecular de Plantas,Centro de Investigación Científica de Yucatán, 2CONACYT, Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingeniería,Universidad Autónoma de Yucatán

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This protocol describes an efficient methodology for the extraction and quantification of caffeine in cell suspensions of C. arabica L. and an experimental process for evaluating the enzymatic activity of caffeine synthase with the expression level of the gene that encodes this enzyme.

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Pech-Kú, R., Muñoz-Sánchez, J. A., Monforte-González, M., Vázquez-Flota, F., Rodas-Junco, B. A., Hernández-Sotomayor, S. T. Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions. J. Vis. Exp. (140), e58166, doi:10.3791/58166 (2018).

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