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An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation
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JoVE Journal 神経科学
An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation

An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation

7,503 Views

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12:26 min

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June 02, 2018

DOI:

10.3791/57857-v

DOI:
10.3791/57857-v

•

12:26 min
June 02, 2018

•

6 Views
,
1Department of Neurobiology,Beijing Institute of Basic Medical Sciences, 2Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration,Nantong University

概要

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This protocol shows how to set up a reliable HFS method in mice. Neurons throughout the hippocampal dentate gyrus are electrically stimulated by HFS directly and indirectly in vivo. Neuronal activity and molecular signaling are examined by c-fos and Notch1 immunofluorescent staining, respectively; neurogenesis is quantified by bromodeoxyuridine labeling assay.

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High-frequency StimulationNeuronal ActivityNeurogenesisHippocampusDeep Brain StimulationMouse Dentate GyrusStereotactic SurgeryElectrode ImplantationBrain Disorders

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Cite this Article

Zhao, Z., Wu, H. An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation. J. Vis. Exp. (136), e57857, doi:10.3791/57857 (2018).

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