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2在 1: 一步亲和纯化为蛋白质蛋白质和蛋白质代谢物的平行的分析

JoVE Journal
生化学

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JoVE Journal 生化学

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

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2在 1: 一步亲和纯化为蛋白质蛋白质和蛋白质代谢物的平行的分析

10,943 Views

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08:23 min

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August 06, 2018

DOI:

10.3791/57720-v

DOI:

10.3791/57720-v

•

08:23 min

August 06, 2018

•

6 Views

Marcin Luzarowski*¹, Izabela Wojciechowska*¹, Aleksandra Skirycz¹

¹Max Planck Institute of Molecular Plant Physiology

概要

蛋白质和蛋白质代谢物相互作用对所有细胞功能都至关重要。在这里, 我们描述了一个协议, 允许并行分析这些交互与蛋白质的选择。我们的协议优化了植物细胞培养, 并结合亲和纯化与质谱为基础的蛋白质和代谢物检测。

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