Here we present a protocol to describe the development and validation of a single molecule array digital ELISA assay, which enables the ultra-sensitive detection of all IFN-α subtypes in human samples.
Llibre, A., Bondet, V., Rodero, M. P., Hunt, D., Crow, Y. J., Duffy, D. Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α. J. Vis. Exp. (136), e57421, doi:10.3791/57421 (2018).