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Imagem de célula viva de segregação do cromossomo durante a mitose
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発生生物学
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JoVE Journal 発生生物学
Live Cell Imaging of Chromosome Segregation During Mitosis

Imagem de célula viva de segregação do cromossomo durante a mitose

9,085 Views

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06:39 min

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March 14, 2018

DOI:

10.3791/57389-v

DOI:
10.3791/57389-v

•

06:39 min
March 14, 2018

•

7 Views
, ,
1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research,US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research,US Food and Drug Administration

概要

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Este protocolo descreve um método fácil e conveniente para rotular e Visualizar ao vivo cromossomos em células mitóticas usando Histone2B-GFP BacMam 2.0 label e um sistema de microscopia confocal de disco girando.

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Cite this Article

Varadkar, P., Takeda, K., McCright, B. Live Cell Imaging of Chromosome Segregation During Mitosis. J. Vis. Exp. (133), e57389, doi:10.3791/57389 (2018).

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