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Imágenes de células vivas de la segregación de cromosomas durante la Mitosis
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発生生物学
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JoVE Journal 発生生物学
Live Cell Imaging of Chromosome Segregation During Mitosis
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Imágenes de células vivas de la segregación de cromosomas durante la Mitosis

9,085 Views

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06:39 min

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March 14, 2018

DOI:

10.3791/57389-v

DOI:
10.3791/57389-v

•

06:39 min
March 14, 2018

•

7 Views
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1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research,US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research,US Food and Drug Administration

概要

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Este protocolo describe un método sencillo y conveniente para marcar y visualizar energizados cromosomas en las células mitotic usando la etiqueta Histone2B-GFP BacMam 2.0 y un sistema de microscopia confocal spinning disc.

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Cite this Article

Varadkar, P., Takeda, K., McCright, B. Live Cell Imaging of Chromosome Segregation During Mitosis. J. Vis. Exp. (133), e57389, doi:10.3791/57389 (2018).

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