We have developed a novel method for co-expressing multiple chimeric fluorescent fusion proteins in plants to overcome the difficulties of conventional methods. It takes advantage of using a single expression plasmid that contains multiple functionally independent protein expressing cassettes to achieve protein co-expression.
Peng, X., Zhong, G., Wang, H. Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants. J. Vis. Exp. (137), e57354, doi:10.3791/57354 (2018).