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Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry
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医学
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JoVE Journal 医学
Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

DOI:
10.3791/57319-v

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07:32 min

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March 06, 2018

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, , , , ,
1内科学科,MUMC, 2CARIM,MUMC, 3Department of Immunology and Biochemistry, Biomedical Research Institute,Hasselt University, 4病理学科,MUMC

章

  • 00:04標題
  • 01:06Stromal Vascular Fraction (SVF) Adipose Tissue (AT) Isolation
  • 02:42SVF Staining and Flow Cytometry Analysis
  • 04:50Results: Representative Visceral AT Immune Cell Abundance
  • 06:33Conclusion

概要

自動翻訳

自動翻訳

This article describes a method to analyze immune cell content of adipose tissue by isolation of immune cells from adipose tissue and subsequent analysis using flow cytometry.

タグ

Flow CytometryStromal Vascular FractionImmune CellsPhenotypingMetabolic ResearchObesityInflammationCell IsolationCell Staining

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