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Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue
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06:14 min
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May 08, 2018
DOI:
1Department of Neurosurgery, Gangnam Severance Hospital,Yonsei University College of Medicine, 2Brain Korea 21 PLUS Project for Medical Science,Yonsei University, 3The Spine and Spinal Cord Institute, Biomedical Center, Gangnam Severance Hospital,Yonsei University College of Medicine, 4Columbia University College of Physicians and Surgeons
概要
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Here, we present two novel methodologies, psPACT and mPACT, for achieving maximal optical transparency and subsequent microscopic analysis of tissue vasculature in the intact rodent whole CNS.
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Woo, J., Lee, E. Y., Park, H., Park, J. Y., Cho, Y. E. Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue. J. Vis. Exp. (135), e57123, doi:10.3791/57123 (2018).
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