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Embriyonik hematopoetik kök hücre öncüleri endotel hücre niş ortak kültürü ile kombine sıralama tek hücre dizini kullanarak klonal analizi
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発生生物学
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JoVE Journal 発生生物学
Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
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Embriyonik hematopoetik kök hücre öncüleri endotel hücre niş ortak kültürü ile kombine sıralama tek hücre dizini kullanarak klonal analizi

8,452 Views

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09:32 min

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May 08, 2018

DOI:

10.3791/56973-v

DOI:
10.3791/56973-v

•

09:32 min
May 08, 2018

•

6 Views
, , , ,
1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Department of Pediatrics, Division of Pediatric Hematology/Oncology,University of Washington School of Medicine

概要

Automatically generated

Burada fare embriyonik gelişim sırasında hematopoetik kök hücre öncüleri klonal analizi için metodoloji mevcut. Endotel hücre ortak kültür ve fenotipik özellikleri ve tek hematopoetik öncüleri potansiyelini engraftment karakterize nakli ile embriyonik aort-erbezi-mesonephros bölgesinden tek hücre dizin sıralama birleştirin.

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Cite this Article

Hadland, B. K., Varnum-Finney, B., Nourigat-Mckay, C., Flowers, D., Bernstein, I. D. Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture. J. Vis. Exp. (135), e56973, doi:10.3791/56973 (2018).

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