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Developmental Biology

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JoVE Journal Developmental Biology
Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
 

Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

Article DOI: 10.3791/56973-v • 09:32 min • May 8th, 2018
May 8th, 2018 •
1,2, 1, 1, 1, 1,2
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Department of Pediatrics, Division of Pediatric Hematology/Oncology, University of Washington School of Medicine

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Here we present methodology for the clonal analysis of hematopoietic stem cell precursors during murine embryonic development. We combine index sorting of single cells from the embryonic aorta-gonad-mesonephros region with endothelial cell co-culture and transplantation to characterize the phenotypic properties and engraftment potential of single hematopoietic precursors.

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Clonal Analysis Embryonic Hematopoietic Stem Cell Precursors Single Cell Index Sorting Endothelial Cell Niche Co-culture Phenotypic Properties Engraftment Potential Developmental Hematopoiesis Hemogenic Precursors Aorta-Gonad-Mesonephros (AGM) 0.25% Collagenase Single Cell Suspension Blocking Buffer
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