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单细胞指数分型与内皮细胞小生境联合培养对胚胎造血干细胞前体的克隆分析
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発生生物学
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JoVE Journal 発生生物学
Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
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单细胞指数分型与内皮细胞小生境联合培养对胚胎造血干细胞前体的克隆分析

8,452 Views

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09:32 min

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May 08, 2018

DOI:

10.3791/56973-v

DOI:
10.3791/56973-v

•

09:32 min
May 08, 2018

•

6 Views
, , , ,
1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Department of Pediatrics, Division of Pediatric Hematology/Oncology,University of Washington School of Medicine

概要

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本文介绍了小鼠胚胎发育过程中造血干细胞前体的克隆分析方法。我们结合内皮细胞共培养和移植对胚胎主动脉-性腺-中区单细胞的指标进行分类, 以表征单造血前体的表型性质和植入电位。

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Cite this Article

Hadland, B. K., Varnum-Finney, B., Nourigat-Mckay, C., Flowers, D., Bernstein, I. D. Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture. J. Vis. Exp. (135), e56973, doi:10.3791/56973 (2018).

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