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Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
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Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

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09:15 min

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January 10, 2018

DOI:

10.3791/56953-v

DOI:
10.3791/56953-v

•

09:15 min
January 10, 2018

•

3 Views
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1Signal Transduction Laboratory, National Institutes of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health (NIH)

概要

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This method describes the cloning, expression, and purification of recombinant Nsa1 for structural determination by X-ray crystallography and small-angle X-ray scattering (SAXS), and is applicable for the hybrid structural analysis of other proteins containing both ordered and disordered domains.

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X-ray CrystallographySmall Angle X-ray Scattering (SAXS)Structural AnalysisProtein StructureRecombinant ProteinUnstructured RegionsNsa1S. CerevisiaeHybrid Structural AnalysisProtein FunctionProtein DynamicsProtein AggregationPurificationAffinity ChromatographyGel FiltrationSDS-PAGETEV ProteaseCentrifugal Filtration

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Lo, Y., Pillon, M. C., Stanley, R. E. Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae. J. Vis. Exp. (131), e56953, doi:10.3791/56953 (2018).

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