ジャーナル
/
免疫学と感染
/
A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

12,657 Views

•

11:40 min

•

January 30, 2018

DOI:

10.3791/56908-v

DOI:
10.3791/56908-v

•

11:40 min
January 30, 2018

•

15 Views
, , , , , , ,
1Dipartimento di Fisica,Università degli Studi di Pavia, 2IRCCS Istituti Clinici Scientifici Maugeri

概要

Automatically generated

We present a protocol to investigate the crosstalk between X-ray-irradiated Caco-2 and peripheral blood mononuclear cells (PBMC). The protocol starts with Caco-2 irradiation and set-up of the co-culture with PBMC; subsequently, trans-epithelial electrical resistance is measured regularly over 48 h and western blot performed in both Caco-2 and PBMC.

Explore More Videos

Caco-2 CellsPBMCsCocultureIonizing RadiationEpithelial Cell PermeabilityTight Junction FunctionRadiation BiologyRadioimmunotherapyCell CultureDensity Gradient CentrifugationX-ray IrradiationDosimetryCell Viability

Read Article

Cite this Article

Babini, G., Morini, J., Barbieri, S., Baiocco, G., Ivaldi, G. B., Liotta, M., Tabarelli de Fatis, P., Ottolenghi, A. A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC. J. Vis. Exp. (131), e56908, doi:10.3791/56908 (2018).

Download .ris file

Copy

Share Video

.

Copy