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Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts
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February 05, 2018
DOI:
1Department of Experimental Medical Science, Wallenberg Neuroscience Center, Division of Neurobiology and Lund Stem Cell Center,Lund University, 2John van Geest Centre for Brain Repair & Department of Neurology, Department of Clinical Neurosciences and Cambridge Stem Cell Institute,University of Cambridge
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Direct neuronal reprogramming generates neurons that maintain the age of the starting somatic cell. Here, we describe a single vector-based method to generate induced neurons from dermal fibroblasts obtained from adult human donors.
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Shrigley, S., Pircs, K., Barker, R. A., Parmar, M., Drouin-Ouellet, J. Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts. J. Vis. Exp. (132), e56904, doi:10.3791/56904 (2018).
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