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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
JoVE Journal
生物学
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JoVE Journal 生物学
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

DOI:
10.3791/56747-v

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10:01 min

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November 28, 2017

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, , , , ,
1Interdisciplinary research center HALOmem,Martin Luther University Halle-Wittenberg, 2化学科,Kings College London

章

  • 00:05標題
  • 00:51Mass Spectrometry-based Protein Identification
  • 04:35Mass Spectrometry of Intact Protein Complexes (Native Mass Spectrometry)
  • 06:17Chemical Cross-linking Coupled with Mass Spectrometry
  • 07:19Results: Chemical Cross-linking and Mass Spectrometry of Multi-subunit Protein Complexes
  • 09:14Conclusion

概要

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自動翻訳

The architecture of protein complexes is essential for their function. Combining various mass spectrometric techniques proved powerful to study their assembly. We provide protocols for chemical cross-linking and native mass spectrometry and show how these complementary techniques help to elucidate the architecture of multi-subunit protein assemblies.

タグ

Chemical Cross-linkingMass SpectrometryIntact Protein ComplexesMulti-subunit Protein AssembliesStructural InformationCompositionConnectivityStoichiometryProtein Complex PurificationElectrophoresisCoomassie StainingProtein Band ExcisionDisulfide ReductionCysteine AlkylationTrypsin DigestionPeptide Extraction

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