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Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System
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JoVE Journal 免疫学と感染
Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System

Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System

9,197 Views

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10:11 min

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January 31, 2018

DOI:

10.3791/56421-v

DOI:
10.3791/56421-v

•

10:11 min
January 31, 2018

•

1 Views
, , , ,
1Division of Infectious Diseases, Department of Medicine and Vancouver Costal Health Research Institute,University of British Columbia, 2Institute of Biomedical Technology,University of Tampere

概要

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A novel technique for rapid antigen display on a bacterial surface is presented, which involves surface biotinylation followed by exposure to proteins of interest in fusion with monomeric avidin. Loading BCG with selected antigens successfully improves its immunogenicity, suggesting that surface decoration can replace traditional genetic approaches.

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Cite this Article

Liao, T. A., Lau, A., Sunil, J., Hytönen, V., Hmama, Z. Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System. J. Vis. Exp. (131), e56421, doi:10.3791/56421 (2018).

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