このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
章
- 00:05標題
- 00:56Overlapping Peptide (OLP) Library Generation and T Cell Priming with OLP Pool-pulsed Dendritic Cells (DCs) Protocol
- 03:43Re-stimulation of OLP Pool-primed CD8+ T Cells Using Macrophages Pulsed with the OLP Pool
- 06:19Results: Identification of a 9-mer Qa-1 Epitope in Myelin Oligodendrocyte Glycoprotein (MOG)
- 07:32Conclusion
Qa-1 (HLA-E in human) belongs to a group of non-classical major histocompatibility complex 1b molecules. Immunization with Qa-1-binding epitopes has been shown to augment tissue-specific immune regulation and ameliorate several autoimmune diseases. Herein we describe an overlapping peptide library strategy for the identification of Qa-1 epitopes in a protein.
