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Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos
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JoVE Journal 免疫学と感染
Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos

Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos

DOI:
10.3791/56352-v

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10:06 min

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August 03, 2017

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, , ,
1Graduate School of Medical Sciences,Kanazawa University

章

  • 00:05標題
  • 00:58Stage 16 Embryo Collection
  • 02:39Embryonic Preparation
  • 04:50Hemocyte and TUNEL Staining
  • 07:41Results: Representative Hemocyte and Apoptotic Cell Staining
  • 09:03Conclusion

概要

自動翻訳

自動翻訳

We herein describe a phagocytosis assay using the dispersed embryonic cells of Drosophila. It enables us to easily and precisely quantify in vivo phagocytosis levels, and to identify new molecules required for the phagocytosis of apoptotic cells.

タグ

PhagocytosisApoptotic CellsDrosophila EmbryosImmune Response発生生物学Cell EngulfmentInflammatory DisordersAutoimmune DiseaseCell IsolationCollagenaseCell SuspensionCell HomogenizationCell Trituration

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