High Resolution Fluorescent <em>In Situ</em> Hybridization in <em>Drosophila</em> Embryos and Tissues Using Tyramide Signal Amplification

Allison Jandura; Jack Hu; Ronit Wilk; Henry M. Krause

doi:10.3791/56281

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High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

JoVE Journal
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High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

12,574 Views

•

14:21 min

•

October 19, 2017

DOI:

10.3791/56281-v

DOI:

10.3791/56281-v

•

14:21 min

October 19, 2017

•

4 Views

Allison Jandura^1,2, Jack Hu¹, Ronit Wilk^1,2, Henry M. Krause^1,2

¹The Terrence Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, ²Department of Molecular Genetics,University of Toronto

概要

The described RNA in situ hybridization protocol allows the detection of RNA in whole Drosophila embryos or dissected tissues. Using 96-well microtiter plates and tyramide signal amplification, transcripts can be detected at high resolution, sensitivity, and throughput, and at a relatively low cost.

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High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

概要

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