Here we describe a facile preparation of chitosan-based injectable hydrogels using dynamic imine chemistry. Methods to adjust the hydrogel’s mechanical strength and its application in 3D cell culture are presented.
Li, Y., Zhang, Y., Wei, Y., Tao, L. Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture. J. Vis. Exp. (127), e56253, doi:10.3791/56253 (2017).