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Analysis of Histone Antibody Specificity with Peptide Microarrays
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JoVE Journal 生化学
Analysis of Histone Antibody Specificity with Peptide Microarrays

Analysis of Histone Antibody Specificity with Peptide Microarrays

DOI:
10.3791/55912-v

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09:47 min

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August 01, 2017

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, ,
1Center for Epigenetics,Van Andel Research Institute

章

  • 00:05標題
  • 01:09Array Slide and Source Plate Layout Design
  • 02:13Microarray Fabrication and Slide Partitioning
  • 04:46Histone Post-translational Modification (PTM) Antibody Hybridization
  • 06:14Data Analysis and Quantification
  • 08:03Results: Analysis of Histone PTM Antibody Specificity with ArrayNinja
  • 08:55Conclusion

概要

自動翻訳

自動翻訳

This manuscript describes methods for applying peptide microarray technology to specificity profiling of antibodies that recognize histones and their post-translational modifications.

タグ

Histone AntibodyPeptide MicroarrayPost-translational ModificationChromatin BiochemistryAntibody SpecificityArray NinjaMicroarray DesignMicroarray PrintingFeature IdentificationSource Plate MapProtein Microarray Printing BufferFluorescein-labeled BiotinMicroarray Printing Software

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