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A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
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August 07, 2017
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Here we present a novel clinical grade isolation and culture method for kidney Perivascular Stromal Cells (kPSCs) based on whole organ perfusion with digestive enzymes and NG2-cell enrichment. With this method, it is possible to acquire sufficient cell numbers for cellular therapy.
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Leuning, D. G., Lievers, E., Reinders, M. E., van Kooten, C., Engelse, M. A., Rabelink, T. J. A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells. J. Vis. Exp. (126), e55841, doi:10.3791/55841 (2017).
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